Antonio G.K. Minero
Antonio is a Ph.D. student at Ruhr-University Bochum working in BioMIP. He graduated from Moscow State University in 2010 with honors. While doing his diploma work, he employed fluorescently labeled DNA in order to investigate conformational changes in complexes of DNA methyltransferases with their DNA substrates. In his current research, he is now involved in chembio ICT and synthetic biology systems, working on electronically controlled DNA replication. His knowledge of fluorescence techniques and biochemistry contributes to the interdisciplinary group research on DNA computing. His research is funded by the European 7th Framework Programme including projects ECCell, MATCHIT and CADMAD.
In ECCell, he implemented an electronically-driven pH-mediated DNA ligation scheme using DNA triplexes in patterned microfluidics. The triplex switch was monitored in a quinhydrone redox system using the FRET-quenching technique. In MATCHIT he made significant progress in DNA immobilization to gold surfaces and contributed to sequence-specific vesicle sorting on a chip in collaboration with the University of Southern Denmark. In CADMAD he is working on DNA construction on a chip and final DNA output separation for downstream gene assembling. He presented his research at Junges Chemie Symposium Ruhr in 2011.
1. Murine DNA methytransferase Dnmt3a: probing of DNA-enzyme interactions with benzo[a]pyrene containing DNA by fluorescence. DECHEMA, 2011.
2. Monitoring and fast analysis of triplex formation. JCS Ruhr, 2011
1. Dimeric bisbenzimidazoles inhibit the DNA methylation catalyzed by the murine Dnmt3a catalytic domain. Cherepanova, N. A.; Ivanov, A. A.; Maltseva, D. V.; Minero, A. S.; Gromyko, A. V.; Streltsov, S. A.; Zhuze, A. L.; Gromova, E. S. Journal of Enzyme Inhibition and Medicinal Chemistry, V. 26, P. 295-300(6), 2011.
2. Mechanism of CpG DNA Methyltransferases M.SssI and Dnmt3a Studied by DNA Containing 2-Aminopurine. Natalia A. Cherepanova, Antonio S. Minero, Alina R. Rakhimova & Elizaveta S. Gromova. Nucleosides, Nucleotides and Nucleic Acids, V. 30, Issue 7-8, 2011.